Cleaved caspase3 抗体
WebSpecificity / Sensitivity. Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb recognizes endogenous levels of mouse caspase-8 only when cleaved at Asp387. In addition to the p18 subunit, this antibody also detects cleavage products containing the pro-domain with the p18 subunit (p43). WebJun 27, 2024 · 划重点,Caspase-3 检测 7 大要点:. 1. 合理的凋亡诱导处理方式:设置药物诱导时间和浓度梯度预实验,以确定特定细胞系中药物诱导 caspase-3 活化的最佳条 …
Cleaved caspase3 抗体
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WebWB analysis of various sample lysates using GTX03281 Caspase 3 (cleaved Asp175) antibody. Lane 1 : HeLa treated with blocking peptide Lane 2 : HeLa (etoposide treated, 25μM 5h) Lane 3 : MCF7 (etoposide treated, 25μM 5h) Lane 4 : Mouse heart tissue lysate Lane 5 : Mouse spleen tissue lysate WebNational Center for Biotechnology Information
WebImmunohistochemistry: Paraffin-embedded Human liver tissue stained with ARG66888 anti-Caspase 3 (cleaved) antibody at 1:200 dilution, overnight at 4°C. Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH … WebAll lanes : Anti-Cleaved Caspase-3 antibody [EPR21032] (ab214430) at 1/5000 dilution Lane 1 : Untreated NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate Lane 2 : NIH/3T3 cells treated with 1 uM staurosprine for 4 hours, whole cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/100000 …
WebSep 13, 2024 · 进一步流式细胞仪及Western blot分析发现,与control组比较,MPP+组细胞凋亡率明显升高(P0.05),Bax、cleaved caspase-3蛋白相对表达量明显升高,Bcl-2蛋白相对表达量明显降低;与MPP+组比较,松果菊苷组细胞凋亡率明显下降(P0.05),Bax、cleaved caspase-3蛋白相对表达量明显降低(P0.05),Bcl ... http://www.ab-mart.com.cn/page.aspx?node=%2077%20&id=%20240805
WebFeb 23, 2024 · caspase-3 和Cleaved-caspase-3 区别在于:caspase-3切割(cleaved)活化之后就会变成Cleaved-caspase-3 ,即后者是前者的活化形式。caspase 是一类与凋亡 …
Web本Caspase-3抗体 (Caspase-3 antibody)为进口分装,用人工合成的人Caspase-3本身的切割位点处一段多肽,经适当修饰后免疫rabbit,然后用protein A和抗原多肽亲和柱经过两步纯化得到的高纯度多克隆抗体。. 本Caspase-3抗体可以识别全长的Caspase-3 (35kD),也可以识别Caspase-3被 ... mfm thanksgiving prayersWebWestern blot: Caspase-3 was detected by western blot analysis with an antibody that recognizes both pro- (32 kD) and active (20 and 17 kD, reflecting the presence or … mfm telehealthhttp://www.bio-review.com/cleaved-caspase-3/ mfm swedish first hillWebPolyclonal Antibody for studying Casp1 (Asp296) cleaved mouse. Cited in 25 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, Cleaved Caspase-1 (Asp296) Antibody … how to calculate corporate taxWebcaspase-3还可以剪切U1-70K、DNA-PK、PKCd 和PKCq 。PKCd 和PKCq 都属于新型PKC(novel PKC, nPKC),当被caspase-3剪切后,可以切除调节区域,而成为活性形式的PKC,另外实验还证明,过量表达PKCd 和PKCq 均可以引起细胞凋亡,说明它们都参与了细胞凋亡的诱导是CTL细胞杀伤机制的重要组成部分。 mfm today serviceWebCleaved Caspase-3 (Asp175) Antibody 可检测 Asp175 附近剪切产生的激活半胱天冬酶-3 的大片段 (17/19 kDa) 的内源水平。该抗体不可检测全长 … how to calculate corporate valueWebCaspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and … how to calculate corrected anion gap