WebJan 31, 2024 · GPX4 uses GSH as an essential cofactor to prevent lipid peroxide and maintain redox homeostasis ( 22 ). In 2008, Seiler et al. identified lipid peroxidation as the key mediator of cell death in glutathione peroxidase 4 (GPX4) knockout cells ( 23 ). Thus, people began to consider this type of cell death different from either apoptosis or necrosis. WebGPx4 deletion reduces keratinocyte adhesion in culture and increases lipid peroxidation and cyclooxygenase-2 (COX-2) levels in cultured keratinocytes and whole skin. Feeding a COX-2 inhibitor to nursing mothers partially prevents development of the abnormal skin phenotype in knockout pups.
Inhibition of tumor propellant glutathione peroxidase 4 induces ...
WebGpx4 −/− HT1080 cells were grown in DMEM supplemented with 10% FBS, 1% glutamine, 1% penicillin-streptomycin mix, 750 μg/ml neomycin. We found that Gpx4-knockout … WebUsing genetic screens, we compared metabolic genes essential for proliferation upon inhibition of cystine uptake or glutathione peroxidase-4 (GPX4). Interestingly, very few genes were commonly required under both conditions, suggesting that cystine limitation and GPX4 inhibition may impair proliferation via distinct mechanisms. ashley lambert kpmg
Oncogenic activation of PI3K-AKT-mTOR signaling …
WebJul 15, 2024 · The study found that silencing of NF2 activates ferroptosis-related pathways in GPX4-knockout tumor mice. NF2-silenced tumor cells grow faster without GPX4 knockout. This is because NF2 knockout facilitates tumor cell metastasis, which GPX4 knockout inhibits. These results indicated that NF2 also depended on GPX4 to regulate … WebJan 4, 2024 · PSTK was associated with the suppression of chemotherapy-induced ferroptosis in HCC cells, and the depletion of PSTK resulted in the inactivation of glutathione peroxidative 4 (GPX4) and the disruption of glutathione (GSH) metabolism owing to the inhibition of selenocysteine and cysteine synthesis, thus enhancing the induction of … WebOct 3, 2016 · GPX4 is a glutathione peroxidase that catalyzes the reduction of lipid peroxides on the cellular membrane. Lipid peroxidation and ferroptosis were observed in mouse xenografts harboring a Gpx4 knockout, highlighting the central regulating role of GPX4 in the ferroptotic pathway . However, we did not observe a change in the level of … ashley kerber