WebMay 3, 2010 · Abstract. Gene 4 protein (gp4) encoded by bacteriophage T7 contains a C-terminal helicase and an N-terminal primase domain. After synthesis of … WebSep 9, 2011 · DNA primase acts as a molecular brake in DNA replication. Nature. 2006; 439:621–624. [Google Scholar] This study uses single-molecule techniques to render real time observation of kinetics of the T7 replication complex. The high ...
Independent and Stochastic Action of DNA Polymerases in the ... - Cell
WebMay 3, 2010 · Abstract. Gene 4 protein (gp4) encoded by bacteriophage T7 contains a C-terminal helicase and an N-terminal primase domain. After synthesis of tetraribonucleotides, gp4 must transfer them to the polymerase for use as primers to initiate DNA synthesis. In vivo gp4 exists in two molecular weight forms, a 56-kDa form and the full-length 63-kDa … Web2006 Nature Publishing Group DNA primase acts as a molecular brake in DNA replication Jong-Bong Lee1, Richard K. Hite2, Samir M. Hamdan1, X. Sunney Xie3, Charles C. … boots pharmacy longwater norwich
Okazaki fragments - Wikipedia
Forked DNA molecules (see Supplementary Information for DNA substrate preparation) are attached by one end to the glass surface of a flow cell and by the other end to a bead (see ref. 14 and Supplementary Information). To prevent nonspecific interactions between the beads and the surface, we … See more T7 gp4 with and without the ZBD, and T7 gp5–trx were purified as previously described10,30. The flow cell containing tethered, forked DNA … See more After particle tracking, traces were corrected for residual instabilities in the flow by subtracting traces corresponding to tethers that were not enzymatically altered. Bead displacements were converted into … See more WebFeb 2, 2006 · In the presence of both leading- and lagging-strand synthesis, we observe the formation and release of a replication loop on the lagging strand. Before loop formation, … WebFeb 2, 2006 · As primer synthesis proceeds, DNA primase acts as a molecular brake on the leading-strand polymerase during slow enzymatic steps on the lagging strand. show all. Associated Content. hat matrix特征值