Roche sirna transfection
WebJan 22, 2024 · A large variety of transfection reagents have been used to deliver DNA and siRNA into cells to achieve genetic manipulations, and may eventually enable non-viral gene therapy. However, it is difficult to optimize the reagents and to achieve favorable results due to its cytotoxicity and stability. WebThrough our Industrial Business, we offer specialty biochemicals for a wide range of industrial applications, as well as custom manufacturing. Explore our comprehensive products and services and learn how Roche can help you make your next discovery. Product families MagNA Pure LightCycler® Digital LightCycler® TIB MolBiol
Roche sirna transfection
Did you know?
WebChoose up to 4 products to compare. Page 1 of 26. As pioneers and current leaders in the field of PCR, Roche nucleic acid purification, PCR and qPCR products provide you with … WebJan 1, 2013 · 5. Step 1 Transfection5.1. Overview. Introduce siRNA at a fixed concentration into cells to silence an mRNA of interest. For a typical single dose screen, siRNAs are transfected at a final concentration of 10 nM to determine maximal knockdown.In this type of transfection, siRNA and the transfection agent are added first followed by the cells – …
WebNov 20, 2015 · The aim of this study was to optimize a lipofection-based small interfering RNA (siRNA) transfection protocol for efficient silencing of gene expression in mature brown adipocytes. We determined that a critical parameter was to deliver the siRNA to mature adipocytes by reverse transfection, i.e. transfection of non-adherent cells. Using … WebMay 16, 2013 · There are myriad ways to introduce siRNA into the cell, from encoding it in plasmids or viruses (generally as a longer RNA that is then processed by the cell) to …
WebsiRNA Details. Gene Symbol: Prkx; Interrogated Sequence Translated Protein Targeted Exon(s) siRNA Location IMAGE Clone ID; RefSeq: NM_016979.1: NP_058675.1: 3 601 GenBank mRNA: AJ238004.1- 3 ... Neon ™ Transfection System 100 µL Kit; WebMar 10, 2013 · siRNA transfection for testing RNA silencing efficiency. ... (150 mM KCl, 25 mM Tris-HCl pH 7.5, 2 mM EDTA, 0.5 mM DTT, 1% NP-40 and Roche Complete Protease Inhibitor Cocktail). Cells were then ...
WebFor transfection of eukaryotic cells with siRNA and miRNA. PolyFect Transfection Reagent. For fast and easy DNA transfection of standard cell lines. Attractene Transfection …
WebROCHE/XTG360-RO - Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines Synonym: transfection reagent Product Type: Chemical X-tremeGENE™ 360 Transfection Reagent (0.4 mL) 1 2 3 4 5 6 7 Prev Next Properties Descriptions Safety Info. Basic Data pictures of gynecomastiaWebRoche x tremegene sirna transfection reagent. X Tremegene Sirna Transfection Reagent, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 … top horrror games on 1.63 ghzWebApr 8, 2024 · A Tmprss6-targeting siRNA that was assayed in parallel showed robust on-target transcript knockdown, confirming successful transfection of all the evaluated … pictures of habitat for humanity homesWebAbout Us. Considered the founder of the industry, Genentech, now a member of the Roche Group, has been delivering on the promise of biotechnology for more than 45 years. … top horse betting sitesWebApr 5, 2024 · 二、Small Interfering RNA Transfection 原文分享 Hepatocytes were seeded into 6-well plates or 96-well plates in Dulbecco’s Modifified Eagle Medium with 10% FBS. Twenty-four hours later, they were transfected with 100 nM of CHOP siRNA or 100 nM of negative control siRNA (siNC) using Advanced Transfection Reagent (Catalog No.: … pictures of habitat for humanityWebReliable Transfection for Common Cell Lines Preserve cell viability Excellent compatibility with everyday cell lines Straightforward protocol; minimal optimization Size 1ml 5 × 1ml 0.5ml Catalog number selected: E2691 $ 460.00 Your price: Log in Add to Cart Add to Helix FuGENE® 6 Transfection Reagent 1ml $ 460.00 Your price: Log in top horse pmuWebFuGENE® 6 Transfection Reagent Part Numbers: E2691, E2692, E2693 Reliable Transfection for Common Cell Lines Preserve cell viability Excellent compatibility with … pictures of gwilym lee