Topo pcr ii dual promotor thermofisher
WebTOPO TA Cloning Dual Promoter kits are for fast, efficient cloning and subsequent in vitro transcription. These kits include the pCRII-TOPO TA vector with dual T7 and SP6 … WebAs far as I know, there are at least five different kits for cloning blunt-end PCR product. 1. NEB PCR Cloning Kit. 2. Thermo fisher CloneJET PCR Cloning Kit 3. Thermo fisher Zero Blunt...
Topo pcr ii dual promotor thermofisher
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WebIntroduction The HTP TOPO TA Cloning® Dual Promoter Kits are specifically designed to simplify high-throughput (HTP) applications. Sufficient pCR ®II-TOPO is provided in bulk … Web2 days ago · Locations of dual-sgRNA target sites were shown in red arrow, primer pair F/R (Mlo2478/2479) were used for PCR testing deletion in flail1 and flail2. B. Selection of red fluorescent seed from T1 plants after transformation of the pKIR1.1-based T-DNA construct to induce the FLAIL2 deletion. Arrows represented selected seeds.
WebSep 4, 2024 · RT-PCR products were either sequenced directly or cloned into the pCRII-TOPO vector with dual promoter (ThermoFisher Scientific) for sequencing. The 5’terminus of the virus was determined using the SMARTer® RACE 5'/3' Kit (Takara Bio USA, Inc., Madison. WI) with the genome-specific primer LN529 ( Table 1 ). WebProduce PCR product Amplify DNA encoding a eukaryotic promoter of choice using Taq polymerase and your own protocol. End the PCR reaction with a final 7 to 10 minute extension step. Perform the TOPO® ® Cloning Reaction 1. Set up one of the following TOPO Cloning reactions using the reagents in the order shown.
WebDual Promoter (pCR®II ) Catalog Numbers K2050-01, K2050-40, K2060-01, K2060-40, K2070-20, and K2070-40 . Document Part Number 250176 Publication Number … WebTOPO TA Cloning Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1). They use a pCR-TOPO Vector with covalently bound topoisomerase I …
WebThey use a pCR-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR-TOPO TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10 Electrocomp™ E. coli
WebThese kits include the pCR™II-TOPO™ TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO™ cloning is the … 黒ニキビ 押し出すWebThey use a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR™-TOPO™ Vectors include: • 3´-T overhangs for direct ligation of … 黒 ニットワンピース ノースリーブWebTOPO™-TA: Content And Storage: Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: tasmanian kitchen pantryWebInvitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10F' Chemically Competent E. coli Designed for cloning PCR products directly from a PCR reaction in just 5 minutes 754.00 EUR - … tasmanian kelpWebLinearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products. Similar to pCR®2.1-TOPO® but with an SP6 promoter. ... Thermo Fisher … 黒 トンボ 俳句WebComments for pCR®II-TOPO® 3973 nucleotides LacZα gene: bases 1-589 M13 Reverse priming site: bases 205-221 Sp6 promoter: bases 239-256 Multiple Cloning Site: bases … tasmanian l2 rulesWebPcrii Topo Dual Promoter Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Average 86 stars, based on 1 Price from $9.99 to $1999.99 tasmanian kingfish